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Vaccination of human volunteers with monovalent and tetravalent live-attenuated dengue vaccine candidates. In contrast, animals that received the YF 17DD vaccine or medium only showed consistent viremia after wild-type D2 virus challenge and seroconverted to D2 thereafter. Please consider upgrading your browser. Genome polyprotein: Specific enzymatic cleavages in vivo yield mature proteins. In the second experiment we expanded the number of flavivirus-naive and YF-immune monkeys. The genome consists of 10, nucleotides. Table 5. Relative evolutionary importance of amino acids within a protein sequence More
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At day 60 all 12 animals were challenged sc with 4.
Group 2 received an sc dose of YF 17DD vaccine 4. Interacts with serine protease NS3 By similarity. Science Since neurotropism is a concern with YF 17D vaccines, a standardized neurovirulence test in monkeys was established to ensure the attenuation of any YF 17D virus 29, Neutralizing antibody titers to D2 were generally lower in the YF pre-immune groups as compared to the YF-naive group, with the exception of monkeys U59 and U Viral Protein Annotation Program.
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Monath T Extracellular Sequence analysis. Microbes Infect. These substrains have been shown to differ in monkey neurovirulence 21but whether they elicit somewhat different anti-YF immune responses in monkeys that would explain the differences in results from the two groups is unclear.
A total of 21 cases of neurologic disease have been reported 2 among millions of vaccinees and encephalitic reactions were also observed in the early development of the YF 17D virus
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May have membrane-destabilizing activity and form viroporins By similarity.
Manual assertion inferred from combination of experimental and computational evidence i. All 6 animals in groups 1 and 2 showed a boost type response after challenge with wild-type D2 virus Table 3. PIR i. Manual assertion according to rules i.
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Mw 17dd 17
|Construction of intertypic chimeric dengue viruses by substitution of structural protein genes. Because YF 17D-dengue chimeric viruses contain the replicative machinery of YF 17D virus and the envelope genes of dengue viruses, their safety should be assessed by the formal neurovirulence test. Clinical signs were observed in only 1 monkey of 10 that received the 17D-D2 virus and were minor.
Taken together, the viremia data after both sc and ic inoculation of monkeys demonstrated limited viral replication, particularly for the chimera.
Monkeys were bled at day 0 and thereafter for 10 consecutive days. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.
News and World Report. 17dd. Peros Banhos Lambrecht,  17dd.
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Salomon Anon., b Lambrecht,Pocklington, Regnaud, Ridley, M. W., Stoddart. 17dd. Peros Banhos Lambrecht,  17dd. Salomon Anon., b Lambrecht,Pocklington, Regnaud, Ridley, M. W., Stoddart.
We examined the ability of the 17D-D2 virus to induce protective immune responses in flavivirus-naive rhesus monkeys. Mature capsid protein C is cleaved at a site upstream of this hydrophobic domain by NS3. Annotation program.
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All 3 animals seroconverted to YF. Capsid protein C.